New Search Search History

Holdings Information

    Molecular microbial diagnostic methods : pathways to implementation for the food and water industries / edited by Nigel Cook, Martin D'Agostino, K. Clive Thompson.

    • Title:Molecular microbial diagnostic methods : pathways to implementation for the food and water industries / edited by Nigel Cook, Martin D'Agostino, K. Clive Thompson.
    •    
    • Other Contributors/Collections:Cook, Nigel, editor.
      D'Agostino, Martin, editor.
      Thompson, K. C. (Kenneth Clive), 1944- editor.
    • Published/Created:London, UK ; San Diego, CA, USA : Academic Press is an imprint of Elsevier, [2016]
    • Holdings

       
    • Library of Congress Subjects:Food--Microbiology--Methodology.
      Water--Microbiology--Methodology.
      Pathogenic microorganisms--Detection.
      Molecular microbiology--Methodology.
    • Medical Subjects: Food Microbiology--methods.
      Water Microbiology.
      Microbiological Techniques.
    • Description:xiii, 251 pages ; 25 cm
    • Summary:Molecular Microbial Diagnostic Methods: Pathways to Implementation in the Food and Water Industries attempts to address the shortage of guidance on implementation of molecular-based methods for routine diagnostic laboratories. What industry and analysts can expect from routine use of these methods is discussed and outlined. The book uncovers industry needs for the use of molecular methods by providing a brief history of water and food analysis for the pathogens of concern. It also describes the potential impact of current and cutting-edge molecular methods. It discusses the advantages of the implementation of molecular methods, describes information on when and how to use specific methods, and presents why one should utilize them for pathogen detection in the routine laboratory. The reference material in the book is also pertinent for anyone carrying out microbiological analysis at the research level and covers a wide spectrum of classical and cutting-edge methods.
    • Notes:Includes bibliographical references and index.
    • ISBN:0124169996 hardcover
      9780124169999 hardcover
    • Contents:Machine generated contents note: ch. 1 Food Industry Current Status
      Introduction
      Molecular Methods Currently Used
      PCR
      Isothermal Techniques
      Sequencing
      Microarrays
      Restriction Enzyme-Based Methods
      Ribotyping
      Laboratory Automation in Molecular Methods
      Challenges
      Sample Preparation
      Limit of Detection
      Live Versus Dead/Infectious Versus Noninfectious
      Interpretation of Results (PCR+/Culture-)
      Acceptance of Use by the Food Industry
      Compliance with Current Legal Requirements
      Disclaimer
      References
      ch. 2 Future Directions for Molecular Microbial Diagnostic Methods for the Food Industry
      Introduction
      Evolution of Food Microbiology Diagnostics: From Petri Dishes to PCR
      Why Introduce an Alternative Molecular Diagnostic Method?
      Time to Final Result
      Discrimination Capacity
      Absence of Reference or Adequate Methods
      Characteristics of an Ideal Food Molecular Method
      Past and Current Challenges
      Past Challenges
      Assessment of the Results and Use of Controls
      Preamplification Treatment of the Food Samples
      Capacity for Quantification
      Determination of Viable Forms of Bacterial Pathogens or Infectious Viral Particles
      Current Challenges
      Simultaneous Detection of Several Foodborne Pathogens
      Harmonization in the Design and Development of a Novel Methodology, and Standardization
      Concluding Remarks
      References
      ch. 3 Current Status of Molecular Microbiological Techniques for the Analysis of Drinking Water
      Introduction and Overview
      Current State of Play
      Influence of Standard and Reference Methods
      Molecular Techniques for Testing Potable Water Quality
      Nucleic Acid Amplification Based Methods
      Cytometry Methods
      Other Molecular Techniques
      Application of Molecular Techniques to Wastewater
      Conclusions
      References
      ch. 4 What is Now Required for Water?
      Introduction and Overview
      Indicator Organisms
      Pathogens in Drinking Water
      Protozoa
      Bacterial Enteric Pathogens
      Bacterial Respiratory Pathogens
      Viruses
      Total Heterotrophic Bacteria
      Feasibility of the Use of Routine Rapid Water Molecular Methods
      Future Predictions
      Conclusions
      References
      ch. 5 CEN/ISO Standards for Both Culture and Molecular Methods
      Introduction
      Standards, Standardization Bodies, and Structures in the Microbiology of the Food Chain
      Definition of the Term "Standard"
      Standardization Bodies at International and European Levels
      Standardization Structures in Microbiology of the Food Chain
      Standards Developed in the Microbiology of the Food Chain
      Standard Reference Methods
      Status of Novel Technologies
      Chromogenic Media
      Molecular Biology Techniques
      Conclusions
      References
      ch. 6 Laboratory Validation, Verification, and Accreditation of Molecular Methods
      Alternative Methods
      Foundations of an Accredited Laboratory
      Expertise: Staff Qualifications and Experience
      Facilities
      Equipment
      Suppliers
      Quality Systems
      Local Quality System
      Method Quality
      Cornerstones of Accreditation
      Validation
      Verification
      Reference Standards/Reference Material
      Proficiency Testing
      Maintaining Accreditation
      Improvement
      Re-verification of the Method
      Internal Auditing
      Following Documented Procedures
      Customer Education
      Performance Characteristics
      Sampling Requirements
      Results Interpretation
      Turnaround Time
      Accreditations
      Health and Safety
      References
      ch. 7 DNA Extraction: Finding the Most Suitable Method
      Boiling Method
      Column Extraction
      Magnetic Beads
      FTA[TM] Cards
      RNA Extraction Methods
      Conclusions
      References
      ch. 8 Assessing Organism Viability and Interpreting Genomic Unit Versus Colony Forming Unit Data for Water and Food Borne Microorganisms, such as Legionella, Campylobacter, Salmonella, and Listeria
      Introduction
      Polymerase Chain Reaction
      Assessing Microbial Viability
      Dead or Alive?
      Viable but Nonculturable Cells (VBNC)
      Importance of Detecting VBNC in Food and Water Samples
      Overcoming the Viability Hurdle
      Pre-PCR Enrichment
      Photoactivatable Chemical Compounds
      Differential Stains
      Immuno-Capture PCR Techniques
      Reverse-Transcription PCR Methods
      When to Use Viability Discrimination
      Standardization and Quality Assurance
      Method Validation/Verification and Interpretation of Results
      Proficiency Testing
      Conclusions
      References
      ch. 9 MALDI-TOF MS: A Rapid Microbiological Confirmation Technique for Food and Water Analysis
      Introduction and Overview
      History and Basis of MALDI-TOF MS and Microbiology
      MALDI-TOF MS for Quick and Reliable Food and Water Analysis
      MALDI-TOF MS and Food Quality Control
      Utilization in Breweries and the Beverage Industry
      Utilization for Monitoring Water in Buildings
      Utilization for Monitoring Other Waters
      Current Confirmation Methods and Advantages of MALDI-TOF MS
      Overview of Current Confirmation Methods
      Conclusions
      MALDI-TOF MS Listeria Speciation Validation
      Introduction
      Validation Project Outline
      Phase 1
      Phase 2
      Phase 3
      Discussion on the Mismatches
      Conclusions and Future Prospects
      References
      ch. 10 Chapter Highlights, Future Requirements, and Conclusions
      Chapter Highlights
      ch. 1 Food Industry Current Status
      ch. 2 Future Directions for Molecular Microbial Diagnostic Methods for the Food Industry
      ch. 3 Current Status of Molecular Microbiological Techniques for the Analysis of Drinking Water
      ch. 4 What is now Required for Water?
      ch. 5 CEN/ISO Standards for Both Culture and Molecular Methods
      ch. 6 Laboratory Validation, Verification, and Accreditation of Molecular Methods
      ch. 7 DNA Extraction: Finding the Most Suitable Method
      ch. 8 Assessing Organism Viability and Interpreting Genomic Unit Versus Colony Forming Unit Data for Water and Food Borne Microorganisms such as Legionella, Campylobacter, Salmonella, and Listeria
      ch. 9 MALDI-TOF MS: A Rapid Microbiological Confirmation Technique for Food and Water Analysis
      Future Requirements and Conclusions
      Fit for Purpose Analysis Improvements
      ISO/IEC 17025 Accreditation Improvements
      Sampling Protocol Improvements
      Sample Preservation, Preparation, and Bacterial Resuscitation Improvements
      Method Validation Protocol Improvements
      Proficiency Testing Improvements
      Microbial Reference Materials Improvements
      Key New Areas
      References.
    Session Timeout
    New Session