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Molecular microbial diagnostic methods : pathways to implementation for the food and water industries / edited by Nigel Cook, Martin D'Agostino, K. Clive Thompson.
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Title:Molecular microbial diagnostic methods : pathways to implementation for the food and water industries / edited by Nigel Cook, Martin D'Agostino, K. Clive Thompson.
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Other Contributors/Collections:Cook, Nigel, editor.
D'Agostino, Martin, editor.
Thompson, K. C. (Kenneth Clive), 1944- editor.
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Published/Created:London, UK ; San Diego, CA, USA : Academic Press is an imprint of Elsevier, [2016]
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Holdings
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Location:WOODWARD LIBRARY stacksWhere is this?
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Call Number: QW25 .M65 2016
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Number of Items:1
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Status:Available
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Location:WOODWARD LIBRARY stacksWhere is this?
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Library of Congress Subjects:Food--Microbiology--Methodology.
Water--Microbiology--Methodology.
Pathogenic microorganisms--Detection.
Molecular microbiology--Methodology.
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Medical Subjects: Food Microbiology--methods.
Water Microbiology.
Microbiological Techniques.
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Description:xiii, 251 pages ; 25 cm
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Summary:Molecular Microbial Diagnostic Methods: Pathways to Implementation in the Food and Water Industries attempts to address the shortage of guidance on implementation of molecular-based methods for routine diagnostic laboratories. What industry and analysts can expect from routine use of these methods is discussed and outlined. The book uncovers industry needs for the use of molecular methods by providing a brief history of water and food analysis for the pathogens of concern. It also describes the potential impact of current and cutting-edge molecular methods. It discusses the advantages of the implementation of molecular methods, describes information on when and how to use specific methods, and presents why one should utilize them for pathogen detection in the routine laboratory. The reference material in the book is also pertinent for anyone carrying out microbiological analysis at the research level and covers a wide spectrum of classical and cutting-edge methods.
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Notes:Includes bibliographical references and index.
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ISBN:0124169996 hardcover
9780124169999 hardcover
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Contents:Machine generated contents note: ch. 1 Food Industry Current Status
Introduction
Molecular Methods Currently Used
PCR
Isothermal Techniques
Sequencing
Microarrays
Restriction Enzyme-Based Methods
Ribotyping
Laboratory Automation in Molecular Methods
Challenges
Sample Preparation
Limit of Detection
Live Versus Dead/Infectious Versus Noninfectious
Interpretation of Results (PCR+/Culture-)
Acceptance of Use by the Food Industry
Compliance with Current Legal Requirements
Disclaimer
References
ch. 2 Future Directions for Molecular Microbial Diagnostic Methods for the Food Industry
Introduction
Evolution of Food Microbiology Diagnostics: From Petri Dishes to PCR
Why Introduce an Alternative Molecular Diagnostic Method?
Time to Final Result
Discrimination Capacity
Absence of Reference or Adequate Methods
Characteristics of an Ideal Food Molecular Method
Past and Current Challenges
Past Challenges
Assessment of the Results and Use of Controls
Preamplification Treatment of the Food Samples
Capacity for Quantification
Determination of Viable Forms of Bacterial Pathogens or Infectious Viral Particles
Current Challenges
Simultaneous Detection of Several Foodborne Pathogens
Harmonization in the Design and Development of a Novel Methodology, and Standardization
Concluding Remarks
References
ch. 3 Current Status of Molecular Microbiological Techniques for the Analysis of Drinking Water
Introduction and Overview
Current State of Play
Influence of Standard and Reference Methods
Molecular Techniques for Testing Potable Water Quality
Nucleic Acid Amplification Based Methods
Cytometry Methods
Other Molecular Techniques
Application of Molecular Techniques to Wastewater
Conclusions
References
ch. 4 What is Now Required for Water?
Introduction and Overview
Indicator Organisms
Pathogens in Drinking Water
Protozoa
Bacterial Enteric Pathogens
Bacterial Respiratory Pathogens
Viruses
Total Heterotrophic Bacteria
Feasibility of the Use of Routine Rapid Water Molecular Methods
Future Predictions
Conclusions
References
ch. 5 CEN/ISO Standards for Both Culture and Molecular Methods
Introduction
Standards, Standardization Bodies, and Structures in the Microbiology of the Food Chain
Definition of the Term "Standard"
Standardization Bodies at International and European Levels
Standardization Structures in Microbiology of the Food Chain
Standards Developed in the Microbiology of the Food Chain
Standard Reference Methods
Status of Novel Technologies
Chromogenic Media
Molecular Biology Techniques
Conclusions
References
ch. 6 Laboratory Validation, Verification, and Accreditation of Molecular Methods
Alternative Methods
Foundations of an Accredited Laboratory
Expertise: Staff Qualifications and Experience
Facilities
Equipment
Suppliers
Quality Systems
Local Quality System
Method Quality
Cornerstones of Accreditation
Validation
Verification
Reference Standards/Reference Material
Proficiency Testing
Maintaining Accreditation
Improvement
Re-verification of the Method
Internal Auditing
Following Documented Procedures
Customer Education
Performance Characteristics
Sampling Requirements
Results Interpretation
Turnaround Time
Accreditations
Health and Safety
References
ch. 7 DNA Extraction: Finding the Most Suitable Method
Boiling Method
Column Extraction
Magnetic Beads
FTA[TM] Cards
RNA Extraction Methods
Conclusions
References
ch. 8 Assessing Organism Viability and Interpreting Genomic Unit Versus Colony Forming Unit Data for Water and Food Borne Microorganisms, such as Legionella, Campylobacter, Salmonella, and Listeria
Introduction
Polymerase Chain Reaction
Assessing Microbial Viability
Dead or Alive?
Viable but Nonculturable Cells (VBNC)
Importance of Detecting VBNC in Food and Water Samples
Overcoming the Viability Hurdle
Pre-PCR Enrichment
Photoactivatable Chemical Compounds
Differential Stains
Immuno-Capture PCR Techniques
Reverse-Transcription PCR Methods
When to Use Viability Discrimination
Standardization and Quality Assurance
Method Validation/Verification and Interpretation of Results
Proficiency Testing
Conclusions
References
ch. 9 MALDI-TOF MS: A Rapid Microbiological Confirmation Technique for Food and Water Analysis
Introduction and Overview
History and Basis of MALDI-TOF MS and Microbiology
MALDI-TOF MS for Quick and Reliable Food and Water Analysis
MALDI-TOF MS and Food Quality Control
Utilization in Breweries and the Beverage Industry
Utilization for Monitoring Water in Buildings
Utilization for Monitoring Other Waters
Current Confirmation Methods and Advantages of MALDI-TOF MS
Overview of Current Confirmation Methods
Conclusions
MALDI-TOF MS Listeria Speciation Validation
Introduction
Validation Project Outline
Phase 1
Phase 2
Phase 3
Discussion on the Mismatches
Conclusions and Future Prospects
References
ch. 10 Chapter Highlights, Future Requirements, and Conclusions
Chapter Highlights
ch. 1 Food Industry Current Status
ch. 2 Future Directions for Molecular Microbial Diagnostic Methods for the Food Industry
ch. 3 Current Status of Molecular Microbiological Techniques for the Analysis of Drinking Water
ch. 4 What is now Required for Water?
ch. 5 CEN/ISO Standards for Both Culture and Molecular Methods
ch. 6 Laboratory Validation, Verification, and Accreditation of Molecular Methods
ch. 7 DNA Extraction: Finding the Most Suitable Method
ch. 8 Assessing Organism Viability and Interpreting Genomic Unit Versus Colony Forming Unit Data for Water and Food Borne Microorganisms such as Legionella, Campylobacter, Salmonella, and Listeria
ch. 9 MALDI-TOF MS: A Rapid Microbiological Confirmation Technique for Food and Water Analysis
Future Requirements and Conclusions
Fit for Purpose Analysis Improvements
ISO/IEC 17025 Accreditation Improvements
Sampling Protocol Improvements
Sample Preservation, Preparation, and Bacterial Resuscitation Improvements
Method Validation Protocol Improvements
Proficiency Testing Improvements
Microbial Reference Materials Improvements
Key New Areas
References.